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 DNA signaturing derived from the internal transcribed spacer 2 (ITS2): a novel tool for identifying Desmodesmus species (Scenedesmaceae, Chlorophyta)
Tác giả hoặc Nhóm tác giả: Minh Ly Nguyen, Xuan Cuong Mai, Nhat Ha Chu, Dang Mau Trinh, Chao-Lin Liu, Chia-Rui Shen
Nơi đăng: Fottea; Số: 23(1);Từ->đến trang: 1-7;Năm: 2023
Lĩnh vực: Nông lâm ngư; Loại: Bài báo khoa học; Thể loại: Quốc tế
TÓM TẮT
The freshwater green algal genus Desmodesmus contains many cryptic species that are difficult to identify using morpho-anatomy. This study analyzed 310 ITS2 sequences related to Desmodesmus species from GenBank and developed a new tool named DNA signaturing. DNA signaturing is a species identification method using signature sequences specific for each species or strain group within the genus Desmodesmus. These signature sequences had 18-33 nucleotides in length. This tool could exactly identify 19 species and 24 strain groups of Desmodesmus. In addition, a signature sequence, AGA GGC TTA AAC TGG GAC C, was specific for almost 90% of Desmodesmus strains and could represent the genus Desmodesmus. A strain MLC1 isolated from freshwater in Da Nang, Vietnam, was identified as Desmodesus pseudoserratus by this tool. DNA signaturing tool described here showed the highest efficacy for identifying strains and a remarkable ability to accurately classify cryptic species in the genus Desmodesmus.
ABSTRACT
The freshwater green algal genus Desmodesmus contains many cryptic species that are difficult to identify using morpho-anatomy. This study analyzed 310 ITS2 sequences related to Desmodesmus species from GenBank and developed a new tool named DNA signaturing. DNA signaturing is a species identification method using signature sequences specific for each species or strain group within the genus Desmodesmus. These signature sequences had 18-33 nucleotides in length. This tool could exactly identify 19 species and 24 strain groups of Desmodesmus. In addition, a signature sequence, AGA GGC TTA AAC TGG GAC C, was specific for almost 90% of Desmodesmus strains and could represent the genus Desmodesmus. A strain MLC1 isolated from freshwater in Da Nang, Vietnam, was identified as Desmodesus pseudoserratus by this tool. DNA signaturing tool described here showed the highest efficacy for identifying strains and a remarkable ability to accurately classify cryptic species in the genus Desmodesmus.
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