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Số người truy cập: 109,894,912
“DNA signaturing” database construction for Tetradesmus species identification and phylogenetic relationships of Scenedesmus-like green microalgae (Scenedesmaceae, Chlorophyta)
Tác giả hoặc Nhóm tác giả:
Xuan Cuong Mai, Chia-Rui Shen, Chao-Lin Liu, Dang Mau Trinh, Minh Ly Nguyen
Nơi đăng:
Journal of Phycology;
S
ố:
59(4);
Từ->đến trang
: 775-784;
Năm:
2023
Lĩnh vực:
Nông lâm ngư;
Loại:
Bài báo khoa học;
Thể loại:
Quốc tế
TÓM TẮT
Species identification of
Scenedesmus
-like microalgae, comprising
Desmodesmus
,
Tetradesmus
, and
Scenedesmus
, has been challenging due to their high morphological and genetic similarity. After developing a DNA signaturing tool for
Desmodesmus
identification, we built a DNA signaturing database for
Tetradesmus
. The DNA signaturing tool contained species-specific nucleotide sequences of
Tetradesmus
species or strain groups with high similarity in ITS2 sequences. To construct DNA signaturing, we collected data on ITS2 sequences, aligned the sequences, organized the data by ITS2 sequence homology, and determined signature sequences according to hemi-compensatory base changes (hCBC)/CBC data from previous studies. Four
Tetradesmus
species and 11 strain groups had DNA signatures. The signature sequence of the genus
Tetradesmus
, TTA GAG GCT TAA GCA AGG ACCC, recognized 86% (157/183) of the collected
Tetradesmus
strains. Phylogenetic analysis of
Scenedesmus
-like species revealed that the
Tetradesmus
species were monophyletic and closely related to each other based on branch lengths.
Desmodesmus
was suggested to split into two subgenera due to their genetic and morphological distinction.
Scenedesmus
must be analyzed along with other genera of the Scenedesmaceae family to determine their genetic relationships. Importantly, DNA signaturing was integrated into a database for identifying
Scenedesmus
-like species through BLAST.
ABSTRACT
Species identification of
Scenedesmus
-like microalgae, comprising
Desmodesmus
,
Tetradesmus
, and
Scenedesmus
, has been challenging due to their high morphological and genetic similarity. After developing a DNA signaturing tool for
Desmodesmus
identification, we built a DNA signaturing database for
Tetradesmus
. The DNA signaturing tool contained species-specific nucleotide sequences of
Tetradesmus
species or strain groups with high similarity in ITS2 sequences. To construct DNA signaturing, we collected data on ITS2 sequences, aligned the sequences, organized the data by ITS2 sequence homology, and determined signature sequences according to hemi-compensatory base changes (hCBC)/CBC data from previous studies. Four
Tetradesmus
species and 11 strain groups had DNA signatures. The signature sequence of the genus
Tetradesmus
, TTA GAG GCT TAA GCA AGG ACCC, recognized 86% (157/183) of the collected
Tetradesmus
strains. Phylogenetic analysis of
Scenedesmus
-like species revealed that the
Tetradesmus
species were monophyletic and closely related to each other based on branch lengths.
Desmodesmus
was suggested to split into two subgenera due to their genetic and morphological distinction.
Scenedesmus
must be analyzed along with other genera of the Scenedesmaceae family to determine their genetic relationships. Importantly, DNA signaturing was integrated into a database for identifying
Scenedesmus
-like species through BLAST.
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