Thông tin chung
  English
  Đề tài NC khoa học
  Bài báo, báo cáo khoa học
  Hướng dẫn Sau đại học
  Sách và giáo trình
  Các học phần và môn giảng dạy
  Giải thưởng khoa học, Phát minh, sáng chế
  Khen thưởng
  Thông tin khác
  Tài liệu tham khảo
  Hiệu chỉnh
 
Số người truy cập: 109,874,595

 Exceptional case of Kasha's rule: Emission from higher-lying singlet electron excited states into ground states in coumarin-based biothiol sensing
Tác giả hoặc Nhóm tác giả: Nguyen KhoaHiena1Doan ThanhNhanbc1Won YoungKimd1MaiVan BaybePham Cam Namf Dang UngVangIn-TaekLimhJong SeungKimdDuong TuanQuangbdD
Nơi đăng: Dyes and Pigments; Số: 152;Từ->đến trang: 118-126;Năm: 2018
Lĩnh vực: Khoa học; Loại: Bài báo khoa học; Thể loại: Quốc tế
TÓM TẮT
A coumarin-based fluorescent sensor (7-Acryloxy-4-methylcoumarin, AMC) for biothiols has been studied by a combination of quantum chemical calculation and experimental investigation. The AMC selectively reacts with mercapto biomoleculesvia Michael addition, which can be served as a ratiometric fluorescent sensor for thiol-containing biomolecules. The AMC sensor shows a selectivity towards biothiols with sensitivity in the order of cysteine > glutathione > homocysteine. Moreover, the AMCsensor can detect the cysteine concentration within the intracellular level. The results of the theoretical investigations on the electron excited states show that the lack of overlapping between MOs in transitions of the lower-lying singlet electron excited states and the small energy gap between electron excited states are the cause of fluorescence from the higher-lying singlet electron excited states, as an exception from Kasha's rule. The fluorescence from the higher-lying singlet electron excited states leads to marked fluorescence enhancement at long wavelength in the products of addition reaction of biothiols to AMC sensor.
ABSTRACT
A coumarin-based fluorescent sensor (7-Acryloxy-4-methylcoumarin, AMC) for biothiols has been studied by a combination of quantum chemical calculation and experimental investigation. The AMC selectively reacts with mercapto biomoleculesvia Michael addition, which can be served as a ratiometric fluorescent sensor for thiol-containing biomolecules. The AMC sensor shows a selectivity towards biothiols with sensitivity in the order of cysteine > glutathione > homocysteine. Moreover, the AMCsensor can detect the cysteine concentration within the intracellular level. The results of the theoretical investigations on the electron excited states show that the lack of overlapping between MOs in transitions of the lower-lying singlet electron excited states and the small energy gap between electron excited states are the cause of fluorescence from the higher-lying singlet electron excited states, as an exception from Kasha's rule. The fluorescence from the higher-lying singlet electron excited states leads to marked fluorescence enhancement at long wavelength in the products of addition reaction of biothiols to AMC sensor.
[ https://www.sciencedirect.com/science/article/abs/pii/S0143720817324671 ]
© Đại học Đà Nẵng
 
 
Địa chỉ: 41 Lê Duẩn Thành phố Đà Nẵng
Điện thoại: (84) 0236 3822 041 ; Email: dhdn@ac.udn.vn