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 Selection of Bacillus thuringiensis against Pathogenic Nematodes Attacking Pepper Tree
Tác giả hoặc Nhóm tác giả: My Pham Thi, Trang Le Vu Khanh, Mai Le Thi, Minh Vo Van, Tuan Vo Chau, Ha Do Thu
Nơi đăng: Journal Biotekhnologya, Russia; Số: Vol 36(2020);Từ->đến trang: 57-62;Năm: 2020
Lĩnh vực: Chưa xác định; Loại: Bài báo khoa học; Thể loại: Quốc tế
TÓM TẮT
Nine strains belonging to the Bacillus genus have been isolated from soil samples in Tien Phuoc district, Quang Nam province. They were capable of surviving at 42°C, and the Bl, B4, B7 and B9 strains could produce toxic crystals at this temperature. B7 had the most promising characteristics in terms of spore-forming ability. The result of the 16S rRNA gene sequencing showed that B7 belongs to the Bacillus thuringiensis species, which is known to effectively control root-knot Meloidogyne sp. nematodes attacking pepper tree. This study was aimed at evaluating the inhibitory effect of the bacterial isolate under study on root-knot eggs and juveniles. It was shown that the highest inhibitory activity of the cultures of the bacterial strains under study was observed at their concentration of 109cells/mL; in this case, up to 89.67% of nematode eggs and 100% of juveniles J2 were killed after 10 h of treatment.
ABSTRACT
Nine strains belonging to the Bacillus genus have been isolated from soil samples in Tien Phuoc district, Quang Nam province. They were capable of surviving at 42°C, and the Bl, B4, B7 and B9 strains could produce toxic crystals at this temperature. B7 had the most promising characteristics in terms of spore-forming ability. The result of the 16S rRNA gene sequencing showed that B7 belongs to the Bacillus thuringiensis species, which is known to effectively control root-knot Meloidogyne sp. nematodes attacking pepper tree. This study was aimed at evaluating the inhibitory effect of the bacterial isolate under study on root-knot eggs and juveniles. It was shown that the highest inhibitory activity of the cultures of the bacterial strains under study was observed at their concentration of 109cells/mL; in this case, up to 89.67% of nematode eggs and 100% of juveniles J2 were killed after 10 h of treatment.
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